Mr Biden has been accused of inappropriate touching by multiple women, but this was the first accusation of serious sexual assault to emerge against the Democratic politician.
Mr Biden's camp has denied the allegation made by Tara Reade, who worked for him when he was a senator, but now acquaintances have corroborated Ms Reade's account.
With accusations flying during a presidential election year - the first since the emergence of the #MeToo movement - let's take a look at what exactly Mr Biden is accused of and how he has responded.
Tara Reade, 56, worked as a staff assistant to Mr Biden from 1992-93 when he was a senator for the US state of Delaware.
In recent interviews, she has said that in 1993 her former boss forced her against a wall and put his hands under her shirt and skirt after she delivered him his gym bag.
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